cx3cr1 is expressed by human b lymphocytes and meditates cx3cl1 driven chemotaxis of tonsil centrocytes二分法cx3cl1 cx3cr1所表达的是人类b淋巴细胞和驱动扁桃体中央细胞的趋化作用.pdfVIP

CX CR1 Is Expressed by Human B Lymphocytes and 3 Meditates CX3CL1 Driven Chemotaxis of Tonsil Centrocytes 1. 1. 2 3 1 Anna Corcione *, Elisa Ferretti , Maria Bertolotto , Franco Fais , Lizzia Raffaghello , Andrea 4 3 2 4 5 5 Gregorio , Claudya Tenca , Luciano Ottonello , Claudio Gambini , Glaucia Furtado , Sergio Lira , Vito Pistoia1 1 Laboratory of Oncology, IRCCS G. Gaslini, Genova, Italy, 2 Laboratory of Phagocyte Physiopathology and Inflammation, Department of Internal Medicine, University of Genoa, Genoa, Italy, 3 Human Anatomy Section, Department of Experimental Medicine, University of Genoa, Genoa, Italy, 4 Human Pathology Section, IRCCS G. Gaslini, Genoa, Italy, 5 Immunobiology Center, Mount Sinai School of Medicine, New York, New York, United States of America Abstract Background: Fractalkine/CX CL1, a surface chemokine, binds to CX CR1 expressed by different lymphocyte subsets. Since 3 3 CX CL1 has been detected in the germinal centres of secondary lymphoid tissue, in this study we have investigated CX CR1 3 3 ¨ expression and function in human naıve, germinal centre and memory B cells isolated from tonsil or peripheral blood. Methodology/Principal Findings: We demonstrate unambiguously that highly purified human B cells from tonsil and peripheral blood expressed CX3CR1 at mRNA and protein levels