cxc chemokine receptor 7 (cxcr7) regulates cxcr4 protein expression and capillary tuft development in mouse kidney科学家趋化因子受体7(cxcr7)调节趋化因子受体cxcr4蛋白表达和老鼠肾脏毛细血管丛发展.pdfVIP

CXC Chemokine Receptor 7 (CXCR7) Regulates CXCR4 Protein Expression and Capillary Tuft Development in Mouse Kidney 1 1. 1.¤ 1 2 1 Sammy Haege *, Claudia Einer , Stefanie Thiele , Wiebke Mueller , Sandor Nietzsche , Amelie Lupp , 3 1 1 Fabienne Mackay , Stefan Schulz , Ralf Stumm 1 Institute of Pharmacology and Toxicology, University Hospital Jena, Friedrich Schiller University Jena, Jena, Germany, 2 Electron Microscopy Centre, University Hospital Jena, Friedrich Schiller University Jena, Jena, Germany, 3 Department of Immunology, Monash University, Central Clinical School, Alfred Medical Research and Education Precinct (AMREP), Melbourne, Victoria, Australia Abstract Background: The CXCL12/CXCR4 axis is involved in kidney development by regulating formation of the glomerular tuft. Recently, a second CXCL12 receptor was identified and designated CXCR7. Although it is established that CXCR7 regulates heart and brain development in conjunction with CXCL12 and CXCR4, little is known about the influence of CXCR7 on CXCL12 dependent kidney development. Methodology/Principal Findings: We provided analysis of CXCR7 expression and function in the developing mouse kidney. Using in situ hybridization, we identified CXCR7 mRNA in epithelial cells including podocytes at all nephron stages up to the mature glomerulus. CXCL12 mRNA showed a striking overlap with CXCR7 mRNA in epithelial structures. In addition, CXCL12 was detected in stromal cells and the glomerular tuft. Expression of CXCR4 was complementary to that of CXCR7 as it occurred in mesenchymal cells, outgrowing ureteric buds and glomerular endothelial cells but not in p