differential gene expression changes in children with severe dengue virus infections差异基因表达变化的孩子严重的登革热病毒感染.pdfVIP

Differential Gene Expression Changes in Children with Severe Dengue Virus Infections 1,2. 3. 1 4 2 Martijn D. de Kruif *, Tatty E. Setiati , Albertus T. A. Mairuhu , Penelopie Koraka , Hella A. Aberson , 2 4 2 1 C. Arnold Spek , Albert D. M. E. Osterhaus , Pieter H. Reitsma , Dees P. M. Brandjes , Augustinus Soemantri3, Eric C. M. van Gorp1 1 Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The Netherlands, 2 Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, The Netherlands, 3 Department of Pediatrics, Dr. Kariadi Hospital, Semarang, Indonesia, 4 Institute of Virology, Erasmus Medical Centre, Rotterdam, The Netherlands Abstract Background: The host response to dengue virus infection is characterized by the production of numerous cytokines, but the overall picture appears to be complex. It has been suggested that a balance may be involved between protective and pathologic immune responses. This study aimed to define differential immune responses in association with clinical outcomes by gene expression profiling of a selected panel of inflammatory genes in whole blood samples from children with severe dengue infections. Methodology/Principal Findings: Whole blood mRNA from 56 Indonesian children with severe dengue virus infections was analyzed during early admission and at day 21, 0, 1, and 5–8 after defervescence. Levels were related to baseline levels collected at a 1-month follow-up visit. Processing of mRNA was performed in a single reaction by multiple