differential growth factor adsorption to calvarial osteoblast-secreted extracellular matrices instructs osteoblastic behavior微分生长因子吸附颅顶的osteoblast-secreted细胞外基质指示成骨细胞的行为.pdfVIP

Differential Growth Factor Adsorption to Calvarial Osteoblast-Secreted Extracellular Matrices Instructs Osteoblastic Behavior 1 2 3 1 Archana Bhat , Simeon A. Boyadjiev , Craig W. Senders , J. Kent Leach * 1 Department of Biomedical Engineering, University of California Davis, Davis, California, United States of America, 2 Section of Genetics, Department of Pediatrics, University of California Davis School of Medicine, Sacramento, California, United States of America, 3 Department of Otolaryngology-Head and Neck Surgery, University of California Davis School of Medicine, Sacramento, California, United States of America Abstract Craniosynostosis (CS), the premature ossification of cranial sutures, is attributed to increased osteogenic potential of resident osteoblasts, yet the contribution of the surrounding extracellular matrix (ECM) on osteogenic differentiation is unclear. The osteoblast-secreted ECM provides binding sites for cellular adhesion and regulates the transport and signaling of osteoinductive factors secreted by the underlying dura mater. The binding affinity of each osteoinductive factor for the ECM may amplify or mute its relative effect, thus contributing to the rate of suture fusion. The purpose of this paper was to examine the role of ECM composition derived from calvarial osteoblasts on protein binding and its resultant effect on cell phenotype. We hypothesized that potent osteoinductive proteins present during sutural fusion (e.g., bone morphogenetic protein-2 (BMP-2) and transforming growth factor beta-1 (TGF-b1)) would exhibit distinct differences in binding when exposed to ECMs generated by human calvarial osteoblasts from unaffected control individuals (CI) or CS p