differential gene expression in soybean leaf tissues at late developmental stages under drought stress revealed by genome-wide transcriptome analysis差异基因表达在干旱胁迫下大豆叶片组织在发育后期阶段揭示了全基因组转录组分析.pdfVIP

Differential Gene Expression in Soybean Leaf Tissues at Late Developmental Stages under Drought Stress Revealed by Genome-Wide Transcriptome Analysis 1,2 1 1 3 3 2 Dung Tien Le , Rie Nishiyama , Yasuko Watanabe , Maho Tanaka , Motoaki Seki , Le Huy Ham , 4 5 1 Kazuko Yamaguchi-Shinozaki , Kazuo Shinozaki , Lam-Son Phan Tran * 1 Signaling Pathway Research Unit, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan, 2 National Key Laboratory of Plant Cell Biotechnology and Agricultural Genetics Institute, Vietnamese Academy of Agricultural Science, Hanoi, Vietnam, 3 Plant Genomic Network Research Team, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan, 4 Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan, 5 Gene Discovery Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan Abstract The availability of complete genome sequence of soybean has allowed research community to design the 66 K Affymetrix Soybean Array GeneChip for genome-wide expression profiling of soybean. In this study, we carried out microarray analysis of leaf tissues of soybean plants, which were subjected to drought stress from late vegetative V6 and from full bloom reproductive R2 stages. Our data analyses showed that out of 46093 soybean genes, which were predicted with high confidence among approximately 66000 putative genes, 41059 genes could be assigned with a known function. Using the criteria of a ratio change . = 2 and a q-value,0.05, we identified 1458 and 1818 upregulated and 1582 and 1688 downregulated genes in