differential gene expression profile associated with the abnormality of bone marrow mesenchymal stem cells in aplastic anemia与异常相关的差异基因表达谱的骨髓间充质干细胞在再生障碍性贫血.pdfVIP

Differential Gene Expression Profile Associated with the Abnormality of Bone Marrow Mesenchymal Stem Cells in Aplastic Anemia 1,2 1 1 3 2 2 1 Jianping Li , Shaoguang Yang , Shihong Lu , Hui Zhao , Jianming Feng , Wenqian Li , Fengxia Ma , 1 1 1 1 1 Qian Ren , Bin Liu , Lei Zhang , Yizhou Zheng , Zhong Chao Han * 1 State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China, 2 Department of Hematology, Qinghai Provincial People’s Hospital, Xining, Qinghai, China, 3 Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China Abstract Aplastic anemia (AA) is generally considered as an immune-mediated bone marrow failure syndrome with defective hematopoietic stem cells (HSCs) and marrow microenvironment. Previous studies have demonstrated the defective HSCs and aberrant T cellular-immunity in AA using a microarray approach. However, little is known about the overall specialty of bone marrow mesenchymal stem cells (BM-MSCs). In the present study, we comprehensively compared the biological features and gene expression profile of BM-MSCs between AA patients and healthy volunteers. In comparison with healthy controls, BM-MSCs from AA patients showed aberrant morphology, decreased proliferation and clonogenic potential and increased apoptosis. BM-MSCs from AA patients were susceptible to be induced to differentiate into adipocytes but more difficult to differentiate into osteoblasts. Consiste