differing prevalence and diversity of bacterial species in fetal membranes from very preterm and term labor不同的患病率和胎膜的细菌物种多样性从非常早产和期限劳动.pdfVIP

Differing Prevalence and Diversity of Bacterial Species in Fetal Membranes from Very Preterm and Term Labor 1,2 3 2 1 1 Hannah E. Jones *, Kathryn A. Harris , Malika Azizia , Lindsay Bank , Bernadette Carpenter , John C. 3 1 2 Hartley , Nigel Klein , Donald Peebles 1 Infectious Diseases and Microbiology Unit, Institute of Child Health, London, United Kingdom, 2 Department of Obstetrics and Gynecology, Institute for Womens Health, University College London, London, United Kingdom, 3 Microbiology Department, Camelia Botner Laboratories, Great Ormond Street Hospital, London, United Kingdom Abstract Background: Intrauterine infection may play a role in preterm delivery due to spontaneous preterm labor (PTL) and preterm prolonged rupture of membranes (PPROM). Because bacteria previously associated with preterm delivery are often difficult to culture, a molecular biology approach was used to identify bacterial DNA in placenta and fetal membranes. Methodology/Principal findings: We used broad-range 16S rDNA PCR and species-specific, real-time assays to amplify bacterial DNA from fetal membranes and placenta. 74 women were recruited to the following groups: PPROM ,32 weeks (n = 26; 11 caesarean); PTL with intact membranes ,32 weeks (n = 19; all vaginal birth); indicated preterm delivery ,32 weeks (n = 8; all caesarean); term (n = 21; 11 caesarean). 50% (5/10) of term vaginal deliveries were positive for bacterial DNA. However, little spread was observed through tissues and species diversity was restricted. Minimal bacteria were detected in term elective section or indicated preterm deliveries. Bacterial prevalence was significantly increased in samples from PTL w