development of self-compatible b. rapa by rnai-mediated s locus gene silencing发展self-compatible b·拉伯rnai-mediated s位点基因沉默.pdfVIP

Development of Self-Compatible B. rapa by RNAi- Mediated S Locus Gene Silencing 1 2 1 1 3 Hee-Jeong Jung , Hyo-Jin Jung , Nasar Uddin Ahmed , Jong-In Park , Kwon-Kyoo Kang , 4 5 1 Yoonkang Hur , Yong-Pyo Lim , Ill-Sup Nou * 1 Department of Horticulture, Sunchon National University, Suncheon, Republic of Korea, 2 Department of Horticulture, Jeollanam-do Agricultural Research and Extension Services, Najusi, Republic of Korea, 3 Department of Horticulture, Hankyong National University, Ansung, Republic of Korea, 4 Department of Biology, Chungnam National University, Daejeon, Republic of Korea, 5 Department of Horticulture, Chungnam National University, Daejeon, Republic of Korea Abstract The self-incompatibility (SI) system is genetically controlled by a single polymorphic locus known as the S-locus in the Brassicaceae. Pollen rejection occurs when the stigma and pollen share the same S-haplotype. Recognition of S-haplotype specificity has recently been shown to involve at least two S-locus genes, S-receptor kinase (SRK) and S-locus protein 11 or S locus Cysteine-rich (SP11/SCR) protein. Here, we examined the function of S60, one SP11/SCR allele of B. rapa cv. Osome, using a RNAi-mediated gene silencing approach. The transgenic RNAi lines were highly self-compatible, and this trait was stable in subsequent generations, even after crossing with other commercial lines. These findings also suggested that the resultant self-compatibility could be transferred to commercial cultivars with the desired performances in B. rapa. Citation: Jung H-J, Jung H-J, Ahmed NU, Park J-I, Kang K-K et al. (2012) Development of Self-Compatible B. rapa by RNAi-Mediated S Locus Gene Silencing. PLoS ONE 7(1