deletion mutants of vpg reveal new cytopathology determinants in a picornavirus缺失突变体vpg揭示新的小核糖核酸病毒细胞病理学因素.pdfVIP

Deletion Mutants of VPg Reveal New Cytopathology Determinants in a Picornavirus 1¤ 1,2 ´ 1 1,2 Armando Arias , Celia Perales , Cristina Escarmıs , Esteban Domingo * ´ ´ ´ ´ 1 Departamento de Virologıa y Microbiologıa, Centro de Biologıa Molecular ‘‘Severo Ochoa’’ (CSIC-UAM), Consejo Superior de Investigaciones Cientıficas (CSIC), Campus ´ ´ ´ de Cantoblanco, Madrid, Spain, 2 Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas (CIBERehd), Barcelona, Spain Abstract Background: Success of a viral infection requires that each infected cell delivers a sufficient number of infectious particles to allow new rounds of infection. In picornaviruses, viral replication is initiated by the viral polymerase and a viral-coded protein, termed VPg, that primes RNA synthesis. Foot-and-mouth disease virus (FMDV) is exceptional among picornaviruses in that its genome encodes 3 copies of VPg. Why FMDV encodes three VPgs is unknown. Methodology and Principal Findings: We have constructed four mutant FMDVs that encode only one VPg: either VPg , 1 VPg , or two chimeric versions containing part of VPg and VPg . All mutants, except that encoding only VPg , were 3 1 3