delayed rectifier and a-type potassium channels associated with kv 2.1 and kv 4.3 expression in embryonic rat neural progenitor cells延迟整流和a类型钾离子通道与千伏2.1和4.3 kv表达在胚胎鼠神经祖细胞.pdfVIP

Delayed Rectifier and A-Type Potassium Channels Associated with Kv 2.1 and Kv 4.3 Expression in Embryonic Rat Neural Progenitor Cells Dean O. Smith1¤*, Julie L. Rosenheimer2¤, Ronald E. Kalil3 1 Stem Cell Research Laboratory, University of Hawaii at Manoa, Manoa, Hawaii, United States of America, 2 Department of Anatomy, University of Hawaii at Manoa, Manoa, Hawaii, United States of America, 3 Department of Ophthalmology and Visual Sciences, University of Wisconsin–Madison, Madison, Wisconsin, United States of America Abstract Background: Because of the importance of voltage-activated K+ channels during embryonic development and in cell proliferation, we present here the first description of these channels in E15 rat embryonic neural progenitor cells derived from the subventricular zone (SVZ). Activation, inactivation, and single-channel conductance properties of recorded progenitor cells were compared with those obtained by others when these Kv gene products were expressed in oocytes. Methodology/Principal Findings: Neural progenitor cells derived from the subventricular zone of E15 embryonic rats were cultured under conditions that did not promote differentiation. Immunocytochemical and Western blot assays for nestin expression indicated that almost all of the cells available for recording expressed this intermediate filament protein, which is generally accepted as a marker for uncommitted embryonic neural progenitor cells. However, a very small numbers of the cells expressed GFAP, a marker for astrocytes, O4, a marker for immature oligodendrocytes, and bIII-tubulin, a marker for neurons. Using immunocytochemistry and Western blots, we detected consistently the expression of Kv2.1, and 4.3. In whole-cell mode, we recorded two outward currents, a delayed rectifier and an A-type current. Conclusions/Sign