deletion hotspots in amacr promoter cpg island are cis-regulatory elements controlling the gene expression in the colon删除热点地区amacr发起人cpg岛顺式元素控制基因表达在结肠.pdfVIP

Deletion Hotspots in AMACR Promoter CpG Island Are cis-Regulatory Elements Controlling the Gene Expression in the Colon Xiang Zhang1,2,3, Irwin Leav4,5, Monica P. Revelo6¤, Ranjan Deka1,2,7, Mario Medvedovic1,2, Zhong Jiang4, Shuk-Mei Ho1,2,3,8* 1 Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America, 2 Center for Environmental Genetics, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America, 3 Department of Surgery, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, 4 Department of Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, 5 Department of Pathology and Laboratory Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, 6 Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America, 7 Center for Genome Information, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America, 8 Cancer Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America Abstract Alpha-methylacyl-coenzyme A racemase (AMACR) regulates peroxisomal b-oxidation of phytol-derived, branched-chain fatty acids from red meat and dairy products — suspected risk factors for colon carcinoma (CCa). AMACR was first found overexpressed in prostate cancer but not in benign glands and is now an established diagnostic marker for prostate cancer. Aberrant expression of AMACR was recently reported in Cca; however, little is known about how this gene is abnormally activated in cancer. By using a panel of immunostained-laser-capture-microdissected clinical sa