disrupted lymph node and splenic stroma in mice with induced inflammatory melanomas is associated with impaired recruitment of t and dendritic cells中断淋巴结和脾脏的基质与诱导炎症小鼠黑色素瘤与招聘t细胞和树突细胞的受损.pdfVIP

Disrupted Lymph Node and Splenic Stroma in Mice with Induced Inflammatory Melanomas Is Associated with Impaired Recruitment of T and Dendritic Cells ¨ 1,2,3 1,2,3 1,2,3 1,2,3 1,2,3 Saıdi M. Soudja , Sandrine Henri , Marielle Mello , Lionel Chasson , Amandine Mas , Maria 1,2,3 1,2,3 1,2,3 ˆ 4 Wehbe , Nathalie Auphan-Anezin , Lee Leserman , Benoıt Van den Eynde , Anne-Marie Schmitt-Verhulst1,2,3* ´ ´ ´ 1 Centre d’Immunologie de Marseille-Luminy (CIML), Universite de la Mediterranee, UMR6546, Marseille, France, 2 INSERM, UMR631, Marseille, France, 3 CNRS, UMR6102, ´ Marseille, France, 4 Ludwig Institute for Cancer Research and de Duve Institute, Universite Catholique de Louvain, Brussels, Belgium Abstract Migration of dendritic cells (DC) from the tumor environment to the T cell cortex in tumor-draining lymph nodes (TDLN) is ¨ essential for priming naıve T lymphocytes (TL) to tumor antigen (Ag). We used a mouse model of induced melanoma in which similar oncogenic events generate two phenotypically distinct melanomas to study the influence of tumor-associated inflammation on secondary lymphoid organ (SLO) organization. One tumor promotes inflammatory cytokines, leading to mobilization of immature myeloid cells (iMC) to the tumor and SLO; the other does not