down regulation of a gene for cadherin, but not alkaline phosphatase, associated with cry1ab resistance in the sugarcane borer diatraea saccharalis调节钙粘蛋白的基因,但不是碱性磷酸酶,与甘蔗螟虫cry1ab阻力diatraea saccharalis.pdfVIP

Down Regulation of a Gene for Cadherin, but Not Alkaline Phosphatase, Associated with Cry1Ab Resistance in the Sugarcane Borer Diatraea saccharalis 1 2 1 1 1 2,3 Yunlong Yang , Yu Cheng Zhu *, James Ottea , Claudia Husseneder , B. Rogers Leonard , Craig Abel , Randall Luttrell2, Fangneng Huang1 1 Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, United States of America, 2 Southern Insect Management Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, Mississippi, United States of America, 3 Corn Insects and Crop Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, United States of America Abstract The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins (i.e., Cry1Ab) in South America and the mid-southern region of the United States. Evolution of insecticide resistance in such target pests is a major threat to the durability of transgenic Bt crops. Understanding the pests’ resistance mechanisms will facilitate development of effective strategies for delaying or countering resistance. Alterations in expression of cadherin- and alkaline phosphatase (ALP) have been associated with Bt resistance in several species of pest insects. In this study, neither the activity nor gene regulation of ALP was associated with Cry1Ab resistance in D. saccharalis. Total ALP enzymatic activity was similar between Cry1Ab-susceptible (Cry1Ab-SS) and -resistant (Cry1Ab-RR) strains of D. saccharalis. In additi