down-regulation of hydrogen sulfide biosynthesis accompanies murine interstitial cells of cajal dysfunction in partial ileal obstruction下调的硫化氢合成伴随小鼠在部分回肠阻塞卡哈尔间质细胞的功能障碍.pdfVIP

Down-Regulation of Hydrogen Sulfide Biosynthesis Accompanies Murine Interstitial Cells of Cajal Dysfunction in Partial Ileal Obstruction 1,2 1 1 1 1 3 1 Xin Guo , Xu Huang , Yi-song Wu , Dong-hai Liu , Hong-li Lu , Yong-chul Kim , Wen-xie Xu * 1 Department of Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China, 2 Department of Urology, Affiliated Hospital of Nantong University, Nantong, China, 3 Department of Physiology, Chungbuk National University College of Medicine, Cheongju, Republic of Korea Abstract Purpose: To investigate the role of endogenous hydrogen sulfide (H S) in partial obstruction-induced dysfunction of the 2 interstitial cells of Cajal (ICC) in mice ileum. Materials and Methods: Partial intestinal obstruction was induced surgically in male imprinting control region (ICR) mice. ICC networks were studied by Immunohistochemistry. Electrical activity was recorded by intracellular recording techniques. The expression of ICC phenotype marker c-kit receptor tyrosine kinase (c-kit), membrane binding stem cell factor (mSCF), the endogenous H2S biosynthesis enzymes cystathionine-b-synthase (CBS) and cystathionine-c-lyase (CSE) was studied by Western blotting. The expression of tumor necrosis factor-a (TNF-a) mRNA was observed by using real-time polymerase chain reaction. Results: Partial intestinal obstruction resulted in ICC networks were disrupted above obstruction 14 days after the operation. The slow waves of intestinal smooth muscles in the dilated region were significantly suppressed and their amplitude and frequency were reduced, whilst the re