drug-resistant genotypes and multi-clonality in plasmodium falciparum analysed by direct genome sequencing from peripheral blood of malaria patients耐药基因型和multi-clonality恶性疟原虫的直接从外周血基因组测序分析疟疾病人.pdfVIP

Drug-Resistant Genotypes and Multi-Clonality in Plasmodium falciparum Analysed by Direct Genome Sequencing from Peripheral Blood of Malaria Patients 1. 2. 2,3 2 4 Timothy Robinson , Susana G. Campino , Sarah Auburn , Samuel A. Assefa , Spencer D. Polley , 2 1,2 1,2 2 2 Magnus Manske , Bronwyn MacInnis , Kirk A. Rockett , Gareth L. Maslen , Mandy Sanders , 2 4,5 1,2 5 4,5 Michael A. Quail , Peter L. Chiodini , Dominic P. Kwiatkowski , Taane G. Clark , Colin J. Sutherland * 1 Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom, 2 Wellcome Trust Sanger Institute, Hinxton, United Kingdom, 3 Global Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Australia, 4 Department of Clinical Parasitology, Hospital for Tropical Diseases, London, United Kingdom, 5 Faculties of Infectious and Tropical Diseases and Epidemiology and Population Health, London School of Hygiene Tropical Medicine, London, United Kingdom Abstract Naturally acquired blood-stage infections of the malaria parasite Plasmodium falciparum typically harbour multiple haploid clones. The apparent number of clones observed in any single infection depends on the diversity of the polymorphic markers used for the analysis, and the relative abundance of rare clones, which frequently fail to be detected a