dual role for pilus in adherence to epithelial cells and biofilm formation in streptococcus agalactiae双重角色的菌毛粘附在链球菌agalactiae上皮细胞和生物膜的形成.pdfVIP

Dual Role for Pilus in Adherence to Epithelial Cells and Biofilm Formation in Streptococcus agalactiae 1. 2. 3. ´ 2 1 Yoan Konto-Ghiorghi , Emilie Mairey , Adeline Mallet , Guillaume Dumenil , Elise Caliot , Patrick 1 1 Trieu-Cuot , Shaynoor Dramsi * ´ ´ ` ` ´ ´ 1 Institut Pasteur, Unite de Biologie des Bacteries Pathogenes a Gram-Positif and URA CNRS 2172, Paris, France, 2 Institut National de la Sante et de la Recherche Medicale ´ ´ ´ ´ (INSERM) U570, Universite Paris, Faculte de Medecine Rene Descartes, UMR- S570, Paris, France, 3 Imagopole, Plate-forme de Microscopie Ultrastructurale, Institut Pasteur, Paris, France Abstract Streptococcus agalactiae is a common human commensal and a major life-threatening pathogen in neonates. Adherence to host epithelial cells is the first critical step of the infectious process. Pili have been observed on the surface of several gram- positive bacteria including S. agalactiae. We previously characterized the pilus-encoding operon gbs1479-1474 in strain NEM316. This pilus is composed of three structural subunit proteins: Gbs1478 (PilA), Gbs1477 (PilB), and Gbs1474 (PilC), and its assembly involves two class C sortases (SrtC3 and SrtC4). PilB, the bona fide pilin, is the major component; PilA, the pilus associated adhesin, and PilC, are both accessory proteins incorporated into the pilus backbone. We first addressed the role of the housekeeping sortase A in pilus biogenesis and showed that it is essential for the covalent anc