蜂胶醇提物微胶囊的制备和表征.pdf

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.10 蜂胶醇提物微胶囊的制备及表征 郭夏丽，蓝雅惠，邹艺红，李熊，罗丽萍 （南昌大学生命科学学院，江西南昌 330031） 摘要：微胶囊具有保护物质免受环境条件影响、掩盖异味和颜色、降低毒性、延长挥发性物质的储存时间，延缓或控制囊心物 的释放等功能。本文采用复凝聚技术制备蜂胶醇提物（EEP ）海藻酸钠-壳聚糖微胶囊，利用粒度分析仪、激光共聚焦显微镜、红外 光谱仪和X 射线衍射仪分析、体外释放试验和加速试验等表征微胶囊性状。研究结果表明，制备的 EEP 微胶囊形状为大小均匀球体， 粒径大小为 265±25 nm ，包埋率和载药量分别达到72.80±3.8%和 19.96±2.4%，海藻酸钠和壳聚糖反应形成微胶囊。模拟胃液和肠液 条件下体外释放 8 h，释放率46.60±0.80%；HPLC 分析加速试验 EEP 微胶囊中 7 种物质含量变化为 14.01%~39.68%，显著低于EEP 中37.01%~77.45%。制得的EEP 微胶囊粒径小、稳定性良好、释放率符合肠溶体系要求，对 EEP 中的活性物质具有一定的保护作用。 关键词：蜂胶醇提物；海藻酸钠；壳聚糖；微胶囊 文章篇号：1673-9078(2016)10-226-232 DOI: 10.13982/j.mfst.1673-9078.2016.10.034 Preparation and Characterization of EEP Calcium Alginate-chitosan Microcapsules GUO Xia-li, LAN Ya-hui, ZOU Yi-hong, LI Xiong, LUO Li-ping (School of Life Sciences, Nanchang University, Nanchang 330031, China) Abstract: Microcapsules are capable of protecting substances from harsh environment conditions, covering up odor and color, reducing toxicity, prolonging the storage of volatile substances, and delaying or controlling the release of capsule core materials. Calcium alginate-chitosan microcapsules of the ethanol extract of propolis (EEP) were prepared by complex coacervation and characterized using a particle-size analyzer, confocal laser-scanning microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, a simulated gastrointestinal-tract model, and a temperature-accelerated test. The results showed that EEP microcapsules were spherical, with a mean particle diameter of 265±25 nm, the encapsulation efficiency and loading capacity were 72.80±3.8% and 19.96±2.4%, resp