e-cadherin promotes incorporation of mouse epiblast stem cells into normal development钙粘蛋白促进整合鼠标外胚层干细胞进入正常发展.pdfVIP

E-Cadherin Promotes Incorporation of Mouse Epiblast Stem Cells into Normal Development 1 1,2 1,2 Satoshi Ohtsuka , Satomi Nishikawa-Torikai , Hitoshi Niwa * 1 Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB), Kobe, Japan, 2 CREST (Core Research for Evolutional Science and Technology), Japan Science Technology Agency, Kawaguchi, Japan Abstract Mouse epiblast stem cells (mEpiSCs) are pluripotent stem cells derived from epiblasts of postimplantation mouse embryos. Their pluripotency is distinct from that of mouse embryonic stem cells (mESCs) in several cell biological criteria. One of the distinctions is that mEpiSCs contribute either not at all or at much lower efficiency to chimeric embryos after blastocyst injection compared to mESCs. However, here we showed that mEpiSCs can be incorporated into normal development after blastocyst injection by forced expression of the E-cadherin transgene for 2 days in culture. Using this strategy, mEpiSCs gave rise to live-born chimeras from 5% of the manipulated blastocysts. There were no obvious signs of reprogramming of mEpiSCs toward the mESC-like state during the 2 days after induction of the E-cadherin transgene, suggesting that mEpiSCs possess latent ability to integrate into the normal developmental process as its origin, epiblasts. Citation: Ohtsuka S, Nishikawa-Torikai S, Niwa H (2012) E-Cadherin Promotes Incorporation of Mouse Epiblast Stem Cells into Normal Development. PLoS ONE 7(9): e45220. doi:10.1371/journal.pone.0045220 Editor: Qiang Wu, National University of Singapore, Singapore Received June 25, 2012; Accepted August 14, 2012; Published September 18, 2012 Copyright: 2012 Ohtsuka et al. This is an open-access article distributed u