electrospun plla nanofiber scaffolds and their use in combination with bmp-2 for reconstruction of bone defects实际上电纺丙交脂纳米纤维支架及其使用结合bmp-2骨缺损的重建.pdfVIP

Electrospun PLLA Nanofiber Scaffolds and Their Use in Combination with BMP-2 for Reconstruction of Bone Defects 1. 1. 1 1 1 Markus D. Schofer , Philip P. Roessler , Jan Schaefer , Christina Theisen , Sonja Schlimme , 2 2 3 3 Johannes T. Heverhagen , Maximilian Voelker , Roland Dersch , Seema Agarwal , Susanne Fuchs- 1 ¨ 1 Winkelmann , Jurgen R. J. Paletta * 1 Department of Orthopedics and Rheumatology, University Hospital Marburg, Marburg, Germany, 2 Department of Diagnostic Radiology, University Hospital Marburg, Marburg, Germany, 3 Department of Macromolecular Chemistry, Philipps-University Marburg, Marburg, Germany Abstract Introduction: Adequate migration and differentiation of mesenchymal stem cells is essential for regeneration of large bone defects. To achieve this, modern graft materials are becoming increasingly important. Among them, electrospun nanofiber scaffolds are a promising approach, because of their high physical porosity and potential to mimic the extracellular matrix (ECM). Materials and Methods: The objective of the present study was to examine the impact of electrospun PLLA nanofiber scaffolds on bone formation in vivo, using a critical size rat calvarial defect model. In addition we analyzed whether direct incorporation of bone morphogenetic protein 2 (BMP-2) into nanofibers could enhance the osteoinductivity of the scaffolds. Two critical size calvarial defects (5 mm) were created in the parietal bones of adult male Sprague-Dawley rats. Defects were either (1) left unfilled, or treated with (2) bovine spongiosa, (3) PLLA scaffolds