electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs电穿孔促进记者转基因和病毒粒子引入血吸虫卵.pdfVIP

Electroporation Facilitates Introduction of Reporter Transgenes and Virions into Schistosome Eggs 1,2. 1,3. 1,2 4 1 Kristine J. Kines , Gabriel Rinaldi , Tunika I. Okatcha , Maria E. Morales , Victoria H. Mann , Jose F. 3 1 Tort , Paul J. Brindley * 1 Department of Microbiology, Immunology Tropical Medicine, George Washington University Medical Center, Washington, D.C., United States of America, ´ 2 Department of Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America, 3 Departamento de Genetica, Facultad de ´ Medicina, Universidad de la Republica (UDELAR), Montevideo, Uruguay, 4 Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America Abstract Background: The schistosome egg represents an attractive developmental stage at which to target transgenes because of the high ratio of germ to somatic cells, because the transgene might be propagated and amplified by infecting snails with the miracidia hatched from treated eggs, and because eggs can be readily obtained from experimentally infected rodents. Methods/Findings: We investigated the utility of square wave electroporation to deliver transgenes and other macromolecules including fluorescent (Cy3) short interference (si) RNA molecules, messenger RNAs, and virions into eggs of Schistosoma mansoni. First, eggs were incubated in Cy3-labeled siRNA with and without square wave electroporation. C