elements in the canine distemper virus m 3′ utr contribute to control of replication efficiency and virulence元素犬瘟热病毒m 3u2032utr有助于控制复制效率和毒性.pdfVIP

Elements in the Canine Distemper Virus M 39 UTR Contribute to Control of Replication Efficiency and Virulence 1,2 1 3 1,2 Danielle E. Anderson , Alexandre Castan , Martin Bisaillon , Veronika von Messling * 1 INRS-Institut Armand-Frappier, University of Quebec, Laval, Quebec, Canada, 2 Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, Singapore, Singapore, 3 University of Sherbrooke, Sherbrooke, Quebec, Canada Abstract Canine distemper virus (CDV) is a negative-sense, single-stranded RNA virus within the genus Morbillivirus and the family Paramyxoviridae. The Morbillivirus genome is composed of six transcriptional units that are separated by untranslated regions (UTRs), which are relatively uniform in length, with the exception of the UTR between the matrix (M) and fusion (F) genes. This UTR is at least three times longer and in the case of CDV also highly variable. Exchange of the M-F region between different CDV strains did not affect virulence or disease phenotype, demonstrating that this region is functionally interchangeable. Viruses carrying the deletions in the M 3 9 UTR replicated more efficiently, which correlated with a reduction of virulence, suggesting that overall length as well as specific sequence motifs distributed throughout the region contribute to virulence. Citation: Anderson DE, Castan A, Bisaillon M, von Messling V (2012) Elements in the Canine Distemper Virus M 39 UTR Contribute to Control of Replication Efficiency and Virulence. PLoS ONE 7(2): e31561. doi:10.1371/journal.pone.0031561 Editor: Elankumaran Subbiah, Virginia Polytechnic Institute and State University, United States of America Received November 2, 2011; Accepted Jan