fusion of the endoplasmic reticulum and mitochondrial outer membrane in rats brown adipose tissue activation of thermogenesis by ca2+内质网和线粒体外膜的融合大鼠棕色脂肪组织激活ca2 +的生热作用.pdfVIP

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fusion of the endoplasmic reticulum and mitochondrial outer membrane in rats brown adipose tissue activation of thermogenesis by ca2+内质网和线粒体外膜的融合大鼠棕色脂肪组织激活ca2 +的生热作用.pdf

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fusionoftheendoplasmicreticulumandmitochondrialoutermembraneinratsbrownadiposetissueactivationofthermogenesisbyca2内质网和线粒体外膜的融合大鼠棕色脂肪组织激活ca2的生热作用

Fusion of the Endoplasmic Reticulum and Mitochondrial Outer Membrane in Rats Brown Adipose Tissue: Activation of Thermogenesis by Ca2+ 1 1 1 2 Leopoldo de Meis *, Luisa A. Ketzer , Rodrigo Madeiro da Costa , Ivone Rosa de Andrade , Marlene Benchimol2 ´ ´ ˆ ´ ´ 1 Instituto de Bioquımica Medica, Centro de Ciencias da Saude, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil, 2 Laboratorio de Ultraestrutura Celular, ´ Universidade Santa Ursula, Rio de Janeiro, Brasil Abstract Brown adipose tissue (BAT) mitochondria thermogenesis is regulated by uncoupling protein 1 (UCP 1), GDP and fatty acids. In this report, we observed fusion of the endoplasmic reticulum (ER) membrane with the mitochondrial outer membrane of rats BAT. Ca2+-ATPase (SERCA 1) was identified by immunoelectron microscopy in both ER and mitochondria. This finding led us to test the Ca2+ effect in BAT mitochondria thermogenesis. We found that Ca2+ increased the rate of respiration and heat production measured with a microcalorimeter both in coupled and uncoupled mitochondria, but had no effect on the rate of ATP synthesis. The Ca2+ concentration needed for half-maximal activation varied between 0.08 and 0.11 mM. The activation of respiration was less pronounced than that of heat production. Heat production and ATP synthesis were inhibited by rotenone and KCN. Liver mitochondria have no UCP1 and during respiration synthesize a large amount of ATP, produce little heat, GDP had no effect on mitochondria coupling, Ca2+ strongly inhibited ATP synthesis and had little or no effect on the small