genotyping performance assessment of whole genome amplified dna with respect to multiplexing level of assay and its period of storage基因分型的性能评估的全基因组扩增dna对多路复用水平的测定及其存储的时期.pdfVIP
- 1、本文档共7页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
genotyping performance assessment of whole genome amplified dna with respect to multiplexing level of assay and its period of storage基因分型的性能评估的全基因组扩增dna对多路复用水平的测定及其存储的时期
Genotyping Performance Assessment of Whole Genome
Amplified DNA with Respect to Multiplexing Level of
Assay and Its Period of Storage
1,2 1 1 2 1,2 2
Daniel W. H. Ho , Wai Chi Yiu , Maurice K. H. Yap , Wai Yan Fung , Po Wah Ng , Shea Ping Yip *
1 Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China, 2 Department of Health
Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China
Abstract
Whole genome amplification can faithfully amplify genomic DNA (gDNA) with minimal bias and substantial genome
coverage. Whole genome amplified DNA (wgaDNA) has been tested to be workable for high-throughput genotyping arrays.
However, issues about whether wgaDNA would decrease genotyping performance at increasing multiplexing levels and
whether the storage period of wgaDNA would reduce genotyping performance have not been examined. Using the
Sequenom MassARRAY iPLEX Gold assays, we investigated 174 single nucleotide polymorphisms for 3 groups of matched
samples: group 1 of 20 gDNA samples, group 2 of 20 freshly prepared wgaDNA samples, and group 3 of 20 stored wgaDNA
samples that had been kept frozen at 270uC for 18 months. MassARRAY is a medium-throughput genotyping platform with
reaction chemistry different from those of high-throughput genotyping arrays. The results showed that genotyping
performance (efficiency and accuracy) of freshly prepared wgaDNA was similar to that of gDNA at various multiplexing
levels (17-plex, 21-plex, 28-plex and 36-plex) of the MassARRAY assays. However, compared with gDNA or fr
您可能关注的文档
- genetic interactions between the drosophila tumor suppressor gene ept and the stat92e transcription factor基因之间的相互作用的果蝇肿瘤抑制基因ept和stat92e转录因子.pdf
- genetic loci associated with plasma phospholipid n-3 fatty acids a meta-analysis of genome-wide association studies from the charge consortium基因位点与血浆磷脂n - 3脂肪酸分析的全基因组关联研究财团.pdf
- genetic manipulation of palmitoylethanolamide production and inactivation in saccharomyces cerevisiae在酿酒酵母基因操纵palmitoylethanolamide生产和失活.pdf
- genetic markers enhance coronary risk prediction in men the morgam prospective cohorts遗传标记增强男性冠心病风险预测morgam潜在群体.pdf
- genetic markers of adult obesity risk are associated with greater early infancy weight gain and growth遗传标记的成年肥胖风险与更早期阶段体重增加和经济增长.pdf
- genetic loci involved in antibody response to mycobacterium avium ssp. paratuberculosis in cattle参与鸟结核分枝杆菌抗体反应ssp基因位点。.pdf
- genetic liability to disability pension in women and men a prospective population-based twin study遗传残疾退休金责任在女性和男性潜在人群双胞胎研究.pdf
- genetic mismatches between nuclei and mitochondria make yeast hybrids sterile细胞核与线粒体基因不匹配使酵母混合动力车无菌.pdf
- genetic manipulation of schistosoma haematobium, the neglected schistosome埃及血吸虫基因操纵,被忽视的血吸虫.pdf
- genetic modifiers of mecp2 function in drosophila在果蝇遗传修饰符的mecp2函数.pdf
- 中国行业标准 GM/T 0126-2023HTML密码应用置标语法.pdf
- 《JJF 2121-2024恒转速源校准规范》.pdf
- 餐饮服务中20条处理要点.docx
- 《GM/T 0011-2023可信计算 可信密码支撑平台功能与接口规范》.pdf
- 《JJF 2134-2024旋转流变仪校准规范》.pdf
- JJF 2121-2024恒转速源校准规范.pdf
- 计量规程规范 JJF 2121-2024恒转速源校准规范.pdf
- 《JJF 2118-2024压力式六氟化硫气体密度控制器校验仪校准规范》.pdf
- JJF 2134-2024旋转流变仪校准规范.pdf
- 计量规程规范 JJF 2134-2024旋转流变仪校准规范.pdf
文档评论(0)