human tumor cell proliferation evaluated using manganese-enhanced mri人类肿瘤细胞增殖使用manganese-enhanced mri评估.pdfVIP
- 1、本文档共9页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
human tumor cell proliferation evaluated using manganese-enhanced mri人类肿瘤细胞增殖使用manganese-enhanced mri评估
Human Tumor Cell Proliferation Evaluated Using
Manganese-Enhanced MRI
1,2 1 1 1 1,3
Rod D. Braun *, David Bissig , Robert North , Kerry S. Vistisen , Bruce A. Berkowitz
1 Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan, United States of America, 2 Barbara Ann Karmanos Cancer
Institute, Wayne State University, Detroit, Michigan, United States of America, 3 Kresge Eye Institute, Wayne State University, Detroit, Michigan, United States of America
Abstract
Background: Tumor cell proliferation can depend on calcium entry across the cell membrane. As a first step toward the
development of a non-invasive test of the extent of tumor cell proliferation in vivo, we tested the hypothesis that tumor cell
uptake of a calcium surrogate, Mn2+ [measured with manganese-enhanced MRI (MEMRI)], is linked to proliferation rate in
vitro.
Methodology/Principal Findings: Proliferation rates were determined in vitro in three different human tumor cell lines:
C918 and OCM-1 human uveal melanomas and PC-3 prostate carcinoma. Cells growing at different average proliferation
rates were exposed to 1 mM MnCl2 for one hour and then thoroughly washed. MEMRI R1 values (longitudinal relaxation
rates), which have a positive linear relationship with Mn2+ concentration, were then determined from cell pellets. Cell cycle
distributions were determined using propidium iodide staining and flow cytometry. All three lines showed Mn2+-induced
increases in R1 compared to cells not exposed to Mn2+. C918 and PC-3 cells each showed a significant, positive correlation
between MEMRI R1 values and proliferation rate (p#0.005), while OCM-1 cells showed no significant correlation. Preliminary,
general modeling of these
您可能关注的文档
- histone deacetylases regulate gonadotropin-releasing hormone i gene expression via modulating otx2-driven transcriptional activity组蛋白去乙酰酶抑制剂调节促性腺激素释放激素通过调节otx2-driven我基因表达转录活动.pdf
- histidine-rich glycoprotein can prevent development of mouse experimental glioblastoma富含组氨酸糖蛋白可以防止老鼠实验胶质母细胞瘤的发展.pdf
- histone h2a mono-ubiquitination is a crucial step to mediate prc1-dependent repression of developmental genes to maintain es cell identity组蛋白h2a mono-ubiquitination调解prc1-dependent镇压是一个关键的一步发展基因保持es细胞的身份.pdf
- histone h2a c-terminus regulates chromatin dynamics, remodeling, and histone h1 binding组蛋白h2a糖调节染色质动力学、重构和组蛋白h1绑定.pdf
- hino an approach for inferring hierarchical organization from regulatory networks日野推断来自监管网络的层次组织的方法.pdf
- hippocampal ca3 transcriptome signature correlates with initial precipitating injury in refractory mesial temporal lobe epilepsy海马ca3转录组签名与初始沉淀在耐火内侧颞叶癫痫损伤.pdf
- histone h3 lysine 27 methylation asymmetry on developmentally-regulated promoters distinguish the first two lineages in mouse preimplantation embryos27组蛋白h3赖氨酸甲基化不对称developmentally-regulated推动者区分前两个血统在老鼠胚胎植入前的胚胎.pdf
- histone h3.3 variant dynamics in the germline of caenorhabditis elegans组蛋白h3.3变体动力学生殖系的线虫.pdf
- histone h3 serine 57 and lysine 56 interplay in transcription elongation and recovery from s-phase stress组蛋白h3丝氨酸57和赖氨酸56相互作用在转录延伸和康复s阶段压力.pdf
- histoplasma capsulatum encodes a dipeptidyl peptidase active against the mammalian immunoregulatory peptide, substance p荚膜组织胞浆菌编码dipeptidyl肽酶活性对哺乳动物的免疫调节肽、p物质.pdf
- GB/T 39560.10-2024电子电气产品中某些物质的测定 第10部分:气相色谱-质谱法(GC-MS)测定聚合物和电子件中的多环芳烃(PAHs).pdf
- 中国国家标准 GB/T 39560.10-2024电子电气产品中某些物质的测定 第10部分:气相色谱-质谱法(GC-MS)测定聚合物和电子件中的多环芳烃(PAHs).pdf
- 《GB/T 39560.10-2024电子电气产品中某些物质的测定 第10部分:气相色谱-质谱法(GC-MS)测定聚合物和电子件中的多环芳烃(PAHs)》.pdf
- GB/T 39560.302-2024电子电气产品中某些物质的测定 第3-2部分:燃烧-离子色谱法(C-IC)筛选聚合物和电子件中的氟、氯和溴.pdf
- 中国国家标准 GB/T 39560.2-2024电子电气产品中某些物质的测定 第2部分:拆解、拆分和机械制样.pdf
- 中国国家标准 GB/T 39560.302-2024电子电气产品中某些物质的测定 第3-2部分:燃烧-离子色谱法(C-IC)筛选聚合物和电子件中的氟、氯和溴.pdf
- GB/T 39560.2-2024电子电气产品中某些物质的测定 第2部分:拆解、拆分和机械制样.pdf
- 《GB/T 39560.2-2024电子电气产品中某些物质的测定 第2部分:拆解、拆分和机械制样》.pdf
- 《GB/T 39560.303-2024电子电气产品中某些物质的测定 第3-3部分:配有热裂解/热脱附的气相色谱-质谱法(Py/TD-GC-MS)筛选聚合物中的多溴联苯、多溴二苯醚和邻苯二甲酸酯》.pdf
- 中国国家标准 GB/T 39560.303-2024电子电气产品中某些物质的测定 第3-3部分:配有热裂解/热脱附的气相色谱-质谱法(Py/TD-GC-MS)筛选聚合物中的多溴联苯、多溴二苯醚和邻苯二甲酸酯.pdf
文档评论(0)