no relationship between embryo morphology and successful derivation of human embryonic stem cell lines没有胚胎形态和成功之间的关系推导的人类胚胎干细胞系.pdfVIP

No Relationship between Embryo Morphology and Successful Derivation of Human Embryonic Stem Cell Lines ¨ 1 1,2 1 ¨ 1 2 Susanne Strom *, Kenny Rodriguez-Wallberg , Frida Holm , Rosita Bergstrom , Linda Eklund , Anne- ¨ 1, Outi Hovatta1 Marie Stromberg 1 Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden, 2 Fertility Unit, Karolinska University Hospital, Huddinge, Sweden Abstract Background: The large number (30) of permanent human embryonic stem cell (hESC) lines and additional 29 which did not continue growing, in our laboratory at Karolinska Institutet have given us a possibility to analyse the relationship between embryo morphology and the success of derivation of hESC lines. The derivation method has been improved during the period 2002–2009, towards fewer xeno-components. Embryo quality is important as regards the likelihood of pregnancy, but there is little information regarding likelihood of stem cell derivation. Methods: We evaluated the relationship of pronuclear zygote stage, the score based on embryo morphology and developmental rate at cleavage state, and the morphology of the blastocyst at the time of donation to stem cell research, to see how they correlated to successful establishment of new hESC lines. Results: Derivation of hESC lines succeeded from poor quality and good quality embryos in the same extent. In several blastocysts, no real inner cell mass (ICM) was seen, but permanent well growing hESC lines could be established. One tripronuclear (3PN) zygote, which developed to blastocyst stage, gave origin to a karyotypically normal hESC line. Conclusion: Even v