non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells非侵入性的探索新生儿胃上皮细胞通过上皮细胞脱落.pdfVIP

Non-Invasive Exploration of Neonatal Gastric Epithelium by Using Exfoliated Epithelial Cells 1 2 1 2 ´ ` 1 Bertrand Kaeffer *, Arnaud Legrand , Thomas Moyon , Anne Frondas-Chauty , Helene Billard , Omar 1 1,2 ´ 2 Guzman-Quevedo , Dominique Darmaun , Jean-Christophe Roze ´ 1 Unite Mixte de Recherche-1280, Physiologie des Adaptations Nutritionnelles, Institut National Recherche Agronomique, Nantes, France, 2 University of Nantes, Hospital of Mother and Child, Nantes, France Abstract Background Aims: In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the removal of the nasogastric tube, (2) whether they can be grown in culture in vitro, and (3) whether the physiological state of exfoliated cells expressing H+/K+ -ATPases reflects that of their counterparts remaining in situ at the surface of the gastric epithelium in neonatal rat pups. Methods: In infants, gastric fluid aspirates were collected weekly after birth or every 3 hours over 24-h periods, and related to clinical parameters (Biocollection PROG/09/18). In rat pups submitted to a single fasting/refeeding cycle, we explored circadian exfoliation with the cellular counter-parts in the gland. All samples were analyzed by confocal imaging and Enzyme-Linked Immunosorbent Assay. Results: Epithelial cells were identified by microscopy using membrane-bound anti-H+/K+ ATPases antibody, assessed for nucleus integrity, and the expression