notch mrna expression in drosophila embryos is negatively regulated at the level of mrna 3′ processing切口mrna表达在果蝇胚胎负监管层面的mrna 3u2032处理.pdfVIP

Notch mRNA Expression in Drosophila Embryos Is Negatively Regulated at the Level of mRNA 39 Processing 1 2 1 Andrew K. Shepherd , Ravinder Singh , Cedric S. Wesley * 1 Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, United States of America, 2 Department of Molecular, Cell and Developmental Biology, University of Colorado, Boulder, Colorado, United States of America Abstract Notch receptor regulates differentiation of almost all tissues and organs during animal development. Many mechanisms function at the protein level to finely regulate Notch activity. Here we provide evidence for Notch regulation at an earlier step - mRNA 39 processing. Processing at the Notch consensus polyadenylation site appears by default to be suppressed in Drosophila embryos. Interference with this suppression, by a mutation, results in increased levels of polyadenylated Notch mRNA, excess Notch signaling, and severe developmental defects. We propose that Notch mRNA 39 processing is negatively regulated to limit the production of Notch protein and render it a controlling factor in the generation of Notch signaling. Citation: Shepherd AK, Singh R, Wesley CS (2009) Notch mRNA Expression in Drosophila Embryos Is Negatively Regulated at the Level of mRNA 39 Processing. PLoS ONE 4(11): e8063. doi:10.1371/journal.pone.0008063 ´ ` Editor: Juan Valcarcel, Centre de Regulacio Genomica, Spain Received September 6, 2009; Accepted October 30, 2009; Published November 30, 2009 Copyright: 2009 Shepherd et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium