n-termini of fungal csl transcription factors are disordered, enriched in regulatory motifs and inhibit dna binding in fission yeastn-termini真菌csl转录因子是无序,浓缩在监管图案和裂殖酵母的抑制dna结合.pdfVIP

N-Termini of Fungal CSL Transcription Factors Are Disordered, Enriched in Regulatory Motifs and Inhibit DNA Binding in Fission Yeast ˇ ´ 1,2 1 ´ ˇ ´ 2 3 3 Martin Prevorovsky *, Sophie R. Atkinson , Martina Ptackova , Janel R. McLean , Kathleen Gould , Petr 2 ˇ ˚ 2 ¨ ¨ 1 Folk , Frantisek Puta , Jurg Bahler 1 Department of Genetics, Evolution Environment and UCL Cancer Institute, University College London, London, United Kingdom, 2 Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic, 3 Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America Abstract Background: CSL (CBF1/RBP-Jk/Suppressor of Hairless/LAG-1) transcription factors are the effector components of the Notch receptor signalling pathway, which is critical for metazoan development. The metazoan CSL proteins (class M) can also function in a Notch-independent manner. Recently, two novel classes of CSL proteins, designated F1 and F2, have been identified in fungi. The role of the fungal CSL proteins is unclear, because the Notch pathway is not present in fungi. In fission yeast, the Cbf11 and Cbf12 CSL paralogs play antagonistic roles in cell adhesion and the coordination of cell and nuclear division. Unusually long N-terminal extensions are typical for fungal and invertebrate CSL family members. In this study, we investigate the functional significance of these extended N-termini of CSL proteins. Methodology/Principal Findings: We identify 15 novel CSL family members from 7 fungal species and conduct bi