nuclear pore proteins nup153 and megator define transcriptionally active regions in the drosophila genome核孔蛋白nup153和megator定义果蝇基因组转录活跃地区.pdfVIP

Nuclear Pore Proteins Nup153 and Megator Define Transcriptionally Active Regions in the Drosophila Genome 1. 2. 2. 3 1,2 Juan M. Vaquerizas , Ritsuko Suyama , Jop Kind , Kota Miura , Nicholas M. Luscombe *, Asifa Akhtar2,4* 1 European Bioinformatics Institute, Cambridge, United Kingdom, 2 Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany, 3 Centre for Molecular and Cellular Imaging, European Molecular Biology Laboratory, Heidelberg, Germany, 4 Laboratory of Chromatin Regulation, Max Planck Institute of Immunobiology, Freiburg, Germany Abstract Transcriptional regulation is one of the most important processes for modulating gene expression. Though much of this control is attributed to transcription factors, histones, and associated enzymes, it is increasingly apparent that the spatial organization of chromosomes within the nucleus has a profound effect on transcriptional activity. Studies in yeast indicate that the nuclear pore complex might promote transcription by recruiting chromatin to the nuclear periphery. In higher eukaryotes, however, it is not known whether such regulation has global significance. Here we establish nucleoporins as a major class of global regulators for gene expression in Drosophila melanogaster. Using chromatin-immunoprecipitation combined with microarray hybridisation, we show that Nup153 and Megator (Mtor) bind to 25% of the genome in continuous domains extending 10 kb to 500 kb. These Nucleoporin-Associated Regions (NARs) are dominated by markers for active transcription, including high RNA polymerase II occupancy and histone H4K16 acetylation. RNAi–mediated knock- down of Nup153 alters the expr