oleic acid biosynthesis in plasmodium falciparum characterization of the stearoyl-coa desaturase and investigation as a potential therapeutic target油酸生物合成的恶性疟原虫表征stearoyl-coa desaturase和调查作为一个潜在的治疗目标.pdfVIP

Oleic Acid Biosynthesis in Plasmodium falciparum: Characterization of the Stearoyl-CoA Desaturase and Investigation as a Potential Therapeutic Target 1 2 3 3 3,4 1 Paul Gratraud , Enlli Huws , Brie Falkard , Sophie Adjalley , David A. Fidock , Laurence Berry , 5,6 2 1,7 1,7 William R. Jacobs, Jr. , Mark S. Baird , Henri Vial , Laurent Kremer * ´ 1 Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Universites de Montpellier II et I, CNRS, UMR 5235, case 107, Montpellier, France, 2 School of Chemistry, Bangor University, Bangor, Wales, 3 Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York, United States of America, 4 Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York, United States of America, 5 Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York, United States of America, 6 Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America, 7 INSERM, DIMNP, Montpellier, France Abstract Background: Plasmodium falciparum parasitization of erythrocytes causes a substantial increase in the levels of intracellular fatty acids, notably oleic acid. How parasites acquire this monounsaturated fatty acid has remained enigmatic. Here, we report on the biochemical and enzymatic characterization of stearoyl-CoA desaturase (SCD) in P. falciparum. Methodology/Principal Findings: Metabolic la