response of escherichia coli containing mycobacterial carotene genes to uv radiation响应包含分枝杆菌胡萝卜素基因的大肠杆菌紫外线辐射.pdfVIP

Journal of Biomedicine and Biotechnology • 1:3 (2001) 79–84 • PII. S1110724301000225 • RESEARCH ARTICLE Response of Escherichia coli containing mycobacterial carotene genes to UV radiation Mohamed Houssaini-Iraqui,1* Naima Khamlichi,1 Jamal El Yamani,1 and Nalin Rastogi2 1Biotechnology Laboratory, Sidi Mohammed Ben Abdellah University, FST, P.O. Box 2202, Fez, Morocco 2 Tuberculosis and Mycobacteria unit, Pasteur Institut, P.O. Box 484, Pointe a Pitre Guadeloupe, France The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A+, was partially digested by restriction en- donucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than nonpigmented strain. INTRODUCTION CH2 OPP Carotenoid pigments are largely distributed in nature. FPP They are present in all of the photosynthetic organisms as IPP GGPP synthase well as in some bacteria, fungi, and yeast [1]. Two major bio- logical roles have been assigned to carotenoids in plants and CH2 OPP prokaryotes. In photosynthetic organisms, these pigments are GGPP involved in trapping light energy. A more general role appli- G