role of exogenous nitric oxide donor in treatment of decompensated hemorrhagic shock in normotensive and hypertensive rats外源一氧化氮供体在治疗失代偿性的正常血压和高血压的大鼠出血性休克.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2012, Article ID 365195, 6 pages doi:10.1155/2012/365195 Research Article Role of Exogenous Nitric Oxide Donor in Treatment of Decompensated Hemorrhagic Shock in Normotensive and Hypertensive Rats Majid Khazaei1 and Babak Barmaki2 1 Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81746-73836, Iran 2 Department of Physiology, Zabol University of Medical Sciences, Zabol, Iran Correspondence should be addressed to Majid Khazaei, khazaei@med.mui.ac.ir Received 11 March 2012; Revised 7 April 2012; Accepted 21 April 2012 Academic Editor: Rudi Beyaert Copyright © 2012 M. Khazaei and B. Barmaki. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Introduction. In this study, we investigated the role of exogenous NO donor, sodium nitroprusside (SNP), on hemodynamic responses and survival rate during decompensated hemorrhagic shock in normotensive and hypertensive rat. Methods. Male wistar rats were divided into normotensive and hypertensive groups (n = 12 each). Then, the animals were subjected to decompensated hemorrhagic shock by withdrawing blood until the mean arterial pressure (MAP) reached to 40 mmHg. After the shock period, the animals were randomly assigned to SNP-treated (0.5 mg/kg) and control groups (n = 6 each). MAP and heart rate (HR) were monitored throughout the experiment and 60 min after the administration of drug. Serum NO concentrations were measured. The survival rate was counted during next 72 h. Results. Infusion of SNP caused no significant changes in MAP and HR in normotensive