role of ac-camp-pka cascade in antidepressant action of electroacupuncture treatment in ratsac-camp-pka级联作用电针刺激治疗大鼠的抗抑郁作用.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 932414, 7 pages doi:10.1155/2012/932414 Research Article Role of AC-cAMP-PKA Cascade in Antidepressant Action of Electroacupuncture Treatment in Rats Jian-hua Liu,1 Zhi-feng Wu,2 Jian Sun,1 Li Jiang,3 Shuo Jiang,3 and Wen-bin Fu1 1 Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China 2 Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China 3 The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510405, China Correspondence should be addressed to Wen-bin Fu, forcer01@163.com Received 4 February 2012; Accepted 27 March 2012 Academic Editor: Bing Zhu Copyright © 2012 Jian-hua Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression. The present study was conducted to explore the role of the cAMP cascade in antidepressant action of electroacupuncture (EA) treatment for chronic mild stress (CMS)-induced depression model rats. The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP- PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine. Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor. Consequently, EA has a significant antidepressant treatment in CMS-indu