role of glucose in irs signaling in rat pancreatic islets specific effects and interplay with insulin在国税局信号作用的葡萄糖鼠胰岛细胞特定的影响和相互作用与胰岛素.pdfVIP

Experimental Diab. Res., 5:257–263, 2004 c Copyright Taylor and Francis Inc. ISSN: 1543-8600 print / 1543-8619 online DOI: 10.1080/15438600490905169 Role of Glucose in IRS Signaling in Rat Pancreatic Islets: Specific Effects and Interplay with Insulin Maryline Paris,1 Catherine Bernard-Kargar,1 Jos´e Vilar,2 Nadim Kassis,1 and Alain Ktorza1,3 1 ´ Laboratoire de Physiopathologie de la Nutrition, Universite Paris 7, Paris-France 2 ´ ´ Physiologie et Endocrinologie Cellulaire et Moleculaire Renale, INSERM U356 Paris-France 3IDRS, Suresnes, France different involvement in the control of β-cell growth and We investigated the possible interplay between insulin function. and glucose signaling pathways in rat pancreatic β-cell with a special focus on the role of glucose in IRS signal- Keywords Glucose; Insulin; IRSs Signaling; Pancreatic Islets; ing in vivo. Three groups of rats were constituted by com- In vivo bining simultaneous infusion during 48 h either of glucose and/or insulin, or glucose+diazoxide: Hyperglycemic- Hyperinsulinemic (HGHI), euglycemic-Hyperinsulinemic INTRODUCTION (eGHI), Hyperglycemic-euinsulinemic (HGeI). Control rats According to our current knowledge, signals generated by were infused with 0,9% NaCl. In HGHI and HGeI rats glucose metabolism within pancreatic β-cells are crucial not plasma glucose levels were maintained at 20-22 mmol/l. In eGHI rats, plasma glucose was not different from that of only for insulin synthesis, storage and rele