role of multiparameter analysis of agnors in fna smears of thyroid swellings in differentiating benign and malignant lesions作用的多参数分析甲状腺肿胀fna)涂片的agnors区分良性和恶性病变.pdfVIP

Hindawi Publishing Corporation Pathology Research International Volume 2012, Article ID 908106, 7 pages doi:10.1155/2012/908106 Clinical Study Role of Multiparameter Analysis of AgNORs in FNA Smears of Thyroid Swellings in Differentiating Benign and Malignant Lesions Mohammad Ismail Hossain,1 Md Quamrul Hassan,2 Pradip Bhattacharjee,1 M. Shahab Uddin Ahamad,2 and Zillur Rahman1 1 Department of Pathology, Chittagong Medical College, Chittagong 4203, Bangladesh 2 Department of Pathology, Cox’s Bazar Medical College, Cox’s Bazar 4700, Bangladesh Correspondence should be addressed to Mohammad Ismail Hossain, ismail.tushar@ Received 14 February 2012; Revised 18 April 2012; Accepted 2 May 2012 Academic Editor: Luigi Terracciano Copyright © 2012 Mohammad Ismail Hossain et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. The aim of this study is to assess the role of multiparameter analysis of silver (Ag)-stained nucleolar organizer regions (AgNORs) technique on aspiration smears of thyroid swellings to distinguish between benign and malignant lesions. Materials and Methods. Aspiration smears from 166 cases of thyroid swellings were examined. Diagnosis was confirmed by histology in 61 cases. AgNOR staining was done on FNA smears according to silver-staining protocol proposed by the International Committee for AgNOR quantification. Multiparameter analysis of AgNORs such as mAgNOR, pAgNOR, and AgNOR size grade was done on 50–100 cells under oil immersion lens. Results. AgNOR parameter of benign and malignant thyroid lesions was compared and was found to be statistically significa