ntgnl1 plays an essential role in pollen tube tip growth and orientation likely via regulation of post-golgi traffickingntgnl1花粉管尖端增长中扮演着重要的角色和定位可能通过调节post-golgi贩运.pdfVIP

NtGNL1 Plays an Essential Role in Pollen Tube Tip Growth and Orientation Likely via Regulation of Post- Golgi Trafficking 1,2 1,3 1 1 1 Fanglei Liao , Lu Wang , Li-Bo Yang , Xiongbo Peng , Mengxiang Sun * 1 Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, China, 2 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, China, 3 Biotechnology Department, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China Abstract Background: Tobacco GNOM LIKE 1 (NtGNL1), a new member of the Big/GBF family, is characterized by a sec 7 domain. Thus, we proposed that NtGNL1 may function in regulating pollen tube growth for vesicle trafficking. Methodology/Principal Findings: To test this hypothesis, we used an RNAi technique to down-regulate NtGNL1 expression and found that pollen tube growth and orientation were clearly inhibited. Cytological observations revealed that both timing and behavior of endocytosis was disrupted, and endosome trafficking to prevacuolar compartments (PVC) or multivesicular bodies (MVB) was altered in pollen tube tips. Moreover, NtGNL1 seemed to partially overlap with Golgi bodies, but clearly colocalized with putative late endosome compartments. We also observed that in such pollen tubes, the Golgi apparatus disassembled and fused with the endoplasmic reticulum, indicating abnormal post-Golgi trafficking. During this process, actin organization was also remodeled. Conclusions/Significance: Thus, we revealed that NtGNL1 is essential for pollen tube growth and orientation and it likely functions via stabilizing the structure of the Golgi apparatus and ensuring post-Golgi