novel targets of the cbrabcrc carbon catabolite control system revealed by transcript abundance in pseudomonas aeruginosa新靶点cbrabcrc碳分解产物的控制系统揭示了成绩单铜绿假单胞菌的丰度.pdfVIP

Novel Targets of the CbrAB/Crc Carbon Catabolite Control System Revealed by Transcript Abundance in Pseudomonas aeruginosa 1 2 2 2¤ 2 Elisabeth Sonnleitner *, Martina Valentini , Nicolas Wenner , Feth el Zahar Haichar , Dieter Haas , Karine Lapouge2* 1 Max F. Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr. Bohrgasse 9, Vienna, Austria, 2 Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland Abstract The opportunistic human pathogen Pseudomonas aeruginosa is able to utilize a wide range of carbon and nitrogen compounds, allowing it to grow in vastly different environments. The uptake and catabolism of growth substrates are organized hierarchically by a mechanism termed catabolite repression control (Crc) whereby the Crc protein establishes translational repression of target mRNAs at CA (catabolite activity) motifs present in target mRNAs near ribosome binding sites. Poor carbon sources lead to activation of the CbrAB two-component system, which induces transcription of the small RNA (sRNA) CrcZ. This sRNA relieves Crc-mediated repression of target mRNAs. In this study, we have identified novel targets of the CbrAB/Crc system in P. aeruginosa using transcriptome analysis in combination with a search for CA motifs. We characterized four target genes involved in the uptake and utilization of less preferred carbon sources: estA (secreted esterase), acsA (acetyl-CoA synthetase), bkdR (regulator of branched-chain amino acid catabolism) and aroP2 (aromatic amino acid uptake protein). Evidence for regulation b