nt-probnp levels in saliva and its clinical relevance to heart failure中位数水平以上病人唾液和心力衰竭的临床意义.pdfVIP

NT-ProBNP Levels in Saliva and Its Clinical Relevance to Heart Failure 1 1 2,3 2,3 2,4 Jared Yong Yang Foo , Yunxia Wan , Karam Kostner , Alicia Arivalagan , John Atherton , Justin Cooper-White1,5, Goce Dimeski2,6, Chamindie Punyadeera1,5* 1The Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, Queensland, Australia, 2 School of Medicine, University of Queensland, Brisbane, Queensland, Australia, 3 Department of Cardiology, Mater Adult Hospital, Brisbane, Queensland, Australia, 4 Department of Cardiology, Royal Brisbane and Women’s Hospital, Brisbane, Queensland, Australia, 5 School of Chemical Engineering, University of Queensland, Brisbane, Queensland, Australia, 6 Chemical Pathology, Princess Alexandra Hospital, Brisbane, Queensland, Australia Abstract Background: Current blood based diagnostic assays to detect heart failure (HF) have large intra-individual and inter- individual variations which have made it difficult to determine whether the changes in the analyte levels reflect an actual change in disease activity. Human saliva mirrors the body’s health and well being and ,20% of proteins that are present in blood are also found in saliva. Saliva has numerous advantages over blood as a diagnostic fluid which allows for a non- invasive, simple, and safe sample collection. The aim of our study was to develop an immunoassay to detect NT-proBNP in saliva and to determine if there is a correlation with blood levels. Methods: Saliva samples were collected from healthy volunteers (n = 40) who had no underlying heart conditions and HF patients (n = 45) at rest. Samples were stored at 280uC until analysis. A customised homoge