screening for antiviral activities of isolated compounds from essential oils筛查抗病毒活动从精油分离出的化合物.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 253643, 8 pages doi:10.1093/ecam/nep187 Original Article Screening for Antiviral Activities of Isolated Compounds from Essential Oils 1, 2 ¨ 3 1 Akram Astani, Jurgen Reichling, and Paul Schnitzler 1 Department of Infectious Diseases, Virology, University of Heidelberg, 69120 Heidelberg, Germany 2 Yazd Shahid Sadoghi University of Medical Science, Safaieh, Yazd, Iran 3 Department of Biology, Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Germany Correspondence should be addressed to Paul Schnitzler, paul schnitzler@med.uni-heidelberg.de Received 22 June 2009; Accepted 15 October 2009 Copyright © 2011 Akram Astani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Essential oil of star anise as well as phenylpropanoids and sesquiterpenes, for example, trans-anethole, eugenol, β -eudesmol, farnesol, β -caryophyllene and β -caryophyllene oxide, which are present in many essential oils, were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro. Antiviral activity was analyzed by plaque reduction assays and mode of antiviral action was determined by addition of the drugs to uninfected cells, to the virus prior to infection or to herpesvirus- infected cells. Star anise oil reduced viral infectivity by 99%, phenylpropanoids inhibited HSV infectivity by about 60–80% and sesquiterpenes suppressed herpes virus infection by 40–98%. Both, sta