seldi-tof-ms serum profiling reveals predictors of cardiac mri changes in marathon runnersseldi-tof-ms血清分析揭示了预测心脏mri的马拉松运动员的变化.pdfVIP

Hindawi Publishing Corporation International Journal of Proteomics Volume 2012, Article ID 679301, 9 pages doi:10.1155/2012/679301 Research Article SELDI-TOF-MS Serum Profiling Reveals Predictors of Cardiac MRI Changes in Marathon Runners George D. Wilson,1, 2 Timothy J. Geddes,1 Barbara L. Pruetz,1 Bryan J. Thibodeau,1 Amy Murawka,1 James M. Colar,3 Peter A. McCullough,3 and Justin E. Trivax3 1 Beaumont BioBank, William Beaumont Hospital, Royal Oak, MI 48073, USA 2 Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI 48073, USA 3 Department of Cardiovascular Medicine, William Beaumont Hospital, Royal Oak, MI 48073, USA Correspondence should be addressed to George D. Wilson, george.wilson@ Received 24 April 2012; Accepted 29 June 2012 Academic Editor: Visith Thongboonkerd Copyright © 2012 George D. Wilson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose. To utilize proteomics to discover proteins associated with significant cardiac magnetic resonance imaging (MRI) changes in marathon runners. Methods. Serum from 25 runners was analyzed by surface enhanced laser desorption ionization time-of- flight mass spectrometry (SELDI-TOF-MS). Proteomic profiles were compared in serum samples obtained prior to the race, at the finish line and within 7 hours after race to identify dynamic proteins correlated with cardiac MRI changes. Results. 693 protein/peptide clusters were identified using two ProteinChip surface chemistries and, of these, 116 were significantly different between the three time points. We identified 7 different patterns of protein expression change wit