species identification of marine fishes in china with dna barcoding海洋鱼类的物种鉴定和dna条码技术在中国.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 978253, 10 pages doi:10.1155/2011/978253 Research Article Species Identification of Marine Fishes in China with DNA Barcoding Junbin Zhang College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China Correspondence should be addressed to Junbin Zhang, jbzhang30@163.com Received 10 December 2010; Accepted 27 February 2011 Copyright © 2011 Junbin Zhang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. DNA barcoding is a molecular method that uses a short standardized DNA sequence as a species identification tool. In this study, the standard 652 base-pair region of the mitochondrial cytochrome oxidase subunit I gene (COI) was sequenced in marine fish specimens captured in China. The average genetic distance was 50-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 15.742% among congeners and only 0.319% for intraspecific individuals. There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the genera Pampus in which the introgressive hybridization was detected. High efficiency of species identification was demonstrated in the present study by DNA barcoding. Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies. 1. Introduction across phylogenetically distant animal groups [12, 13]. To