statistical analysis of efficient unbalanced factorial designs for two-color microarray experiments统计分析的效率不平衡的阶乘设计的双色微阵列实验.pdfVIP

Hindawi Publishing Corporation International Journal of Plant Genomics Volume 2008, Article ID 584360, 16 pages doi:10.1155/2008/584360 Review Article Statistical Analysis of Efficient Unbalanced Factorial Designs for Two-Color Microarray Experiments Robert J. Tempelman Department of Animal Science, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI 48824-1225, USA Correspondence should be addressed to Robert J. Tempelman, tempelma@ Received 2 November 2007; Revised 22 January 2008; Accepted 25 April 2008 Recommended by Chunguang Du Experimental designs that efficiently embed a fixed effects treatment structure within a random effects design structure typically require a mixed-model approach to data analyses. Although mixed model software tailored for the analysis of two-color microarray data is increasingly available, much of this software is generally not capable of correctly analyzing the elaborate incomplete block designs that are being increasingly proposed and used for factorial treatment structures. That is, optimized designs are generally unbalanced as it pertains to various treatment comparisons, with different specifications of experimental variability often required for different treatment factors. This paper uses a publicly available microarray dataset, as based upon an efficient experimental design, to demonstrate a proper mixed model analysis of a typical unbalanced factorial design characterized by incomplete blocks and hierarchical levels of variability. Copyright © 2008 Robert J. Tempelman. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.