the components of flemingia macrophylla attenuate amyloid β-protein accumulation by regulating amyloid β-protein metabolic pathway的组件flemingia macrophylla减弱淀粉样β-protein积累通过调节淀粉样β-protein代谢途径.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 795843, 9 pages doi:10.1155/2012/795843 Research Article The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway Yun-Lian Lin,1 Huey-Jen Tsay,2 Yung-Feng Liao,3 Mine-Fong Wu,3 Chuen-Neu Wang,4 and Young-Ji Shiao3, 4 1 Division of Medicinal Chemistry, National Research Institute of Chinese Medicine, Taipei 112, Taiwan 2 Institute of Neuroscience, National Yang-Ming University, Taipei 112, Taiwan 3 Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei 112, Taiwan 4 Division of Basic Chinese Medicine, National Research Institute of Chinese Medicine, Taipei 112, Taiwan Correspondence should be addressed to Young-Ji Shiao, yshiao@.tw Received 10 January 2012; Revised 6 March 2012; Accepted 13 March 2012 Academic Editor: Karl Wah-Keung Tsim Copyright © 2012 Yun-Lian Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Flemingia macrophylla (Leguminosae) is a popular traditional remedy used in Taiwan as anti-inflammatory, promoting blood circulation and antidiabetes agent. Recent study also suggested its neuroprotective activity against Alzheimer’s disease. Therefore, the effects of F. macrophylla on Aβ production and degradation were studied. The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells). The effects on Aβ degradation were evaluated on a cell-free system. An ELISA assay was applied t