the effect of glycyrrhetinic acid on pharmacokinetics of cortisone and its metabolite cortisol in rats可的松的甘草次酸对药物动力学的影响及其代谢物皮质醇的老鼠.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2012, Article ID 856324, 5 pages doi:10.1155/2012/856324 Research Article The Effect of Glycyrrhetinic Acid on Pharmacokinetics of Cortisone and Its Metabolite Cortisol in Rats Dan Lin,1 Wei Sun,2, 3 Zhe Wang,3 Lian-Guo Chen,3 Xiao-Le Chen,3 Shuang-Hu Wang,3 Wan-Shu Li,3 Ren-Shan Ge,2 and Guo-Xin Hu3, 4 1Analytical and Testing Center, Wenzhou Medical College, Wenzhou 325035, China 2 The 2nd Affi liated Hospital Wenzhou Medical College, Wenzhou 325027, China 3 School of Pharmacy, Wenzhou Medical College, Wenzhou 325035, China 4 Teaching Center of Medical Functional Experiment, Wenzhou Medical College, Wenzhou 325035, China Correspondence should be addressed to Guo-Xin Hu, wzhhgx@ Received 2 July 2012; Revised 1 October 2012; Accepted 2 October 2012 Academic Editor: James D. Murray Copyright © 2012 Dan Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The purpose of this paper is to study pharmacokinetics of cortisone (E) and its metabolite cortisol (F) in rats after administration of glycyrrhetinic acid (GA) and cortisone. Healthy male SD rats were randomized to be given 20 mg/kg E or E combined with 10 mg/kg GA. Blood samples were collected at 5, 10, 20, 40, 60, 90, 120, 150, 180, and 240 min after administration. The serum concentrations of E and F were determined by HLPC and pharmacokinetic parameters were calculated using DASver2.0 software. The parameters of AUC(0−t) , AUC(0−∞) , and Cmax for E in the group of E + GA were significantly higher than those in the group of E (P 0.01); the half-time (t1/ 2β