百合杂种系间杂交障碍的克服和3种幼胚离体培养方法的比较研究_谢松林.pdfVIP

, 2010, 30( 8) : 1573- 1578 A cta Bot. Bor eal .-Occid ent . Sin . ¹ : 1000-4025( 2010) 08- 1573-06 3 1 2 1 2* 谢松林 , 王仙芝 , 牛立新 , 张延龙 ( 1 , 712100; 2 , 712100) : ( Longiflorum, L)ö White Fo ø ( Asiat ic, A)ö Conneticut Kingø ( Orien- tal, O)ö Sorbonneø ( Trumpet, T )ö Roy al Godenø , , , . , 6 , LL @ TT 5 ; 3 100% 21. 5% 1% , 9. 6% 89. 9% 0. 5% ; , . , , , . : ; ; ; ; ; ( GISH) : Q813. 7 : A Overcoming Cross Barriers and Obtaining Crossing Hybrids between Different Hybrid Groups of Lily (Lilium) 1 2 1 2* XIE Song-lin , WANG Xian-zhi , N IU L-i in , ZHANG Yan-long ( 1 College of H orticulture, Northw est A F U niversity, Yangling, Shaan i 712100, China; 2 College of Forestry, Northwest A F U niversity, Yangling, Shaan i 712100, Chin a) Abstract Inter specific hybridization w as undertaken betw een lily cultivar s from different hybrid gr oups, viz.ö W hite Fo ø from Longiflorum,ö Conneticut Kingø from Asiatic,ö Sorbonneø from Oriental and ö Royal Godenø from T rumpet, cut-style pollination method and embryo rescue technique w ere used to overcom hy- bridization barrier s, and the hybr ids w ere identified by genomic in s itu hybridization ( GISH ) . The r esults show ed that cut-style pollination significantly incr eased the crossing efficiency e cept the LL @ T T comb-i nation, the efficiency of embryo, embryo sac and ovule culture w ere 100%, 22% and 1% respectively, and 9. 6% , 89. 9% and 0. 5% of the progeny w ere finally obtained. Genomic in s it u hybr