879-基因工程（879- gene engineering）.docVIP

879-基因工程（879- gene engineering） Genetic engineering (2011 Jiangsu volume) 33. (8 points), please answer the questions concerning genetic engineering: (1) using PCR technology to amplify target genes, the principle is similar to that of intracellular DNA replication (as shown below). The primer is a single stranded DNA fragment, which is the basis for the synthesis and elongation of daughter strands. From the theory that fourth cycle of products containing primer A DNA fragment of the proportion of 3. It began to appear two dideoxy nucleotide chain length DNA fragment in the long cycle of products. (2) designing primers is one of the key steps of PCR technology. The two sets of primers designed by a student (with only partial base sequences) are not reasonable (as below), please explain the reasons. The first groups: the second group: ^ ^. (3) PCR reaction system containing thermostable DNA polymerase, the following expressions can not correctly reflect the DNA polymerase function, this is because . (4) cut the same plasmid individually or in combination with restriction enzymes EcoRV and Mbol, and obtain the length of the DNA fragment as follows (1 KB) That is, 1000 base pairs, please specify the cutting sites of EcoRV and Mbol on the plasmid at the specified location of the answer sheet. 33. (8 points) (1) 15/16, II. Three (2) the primer pairs I and primer II partially fail to complement each other When the primer Ifolds itself, the local base pairing occurs and the complement fails (3) the DNA polymerase can only bind a single deoxyribonucleic acid Pairs of primer strands on double stranded DNA segments (4) see the right picture (11 year Beijing volume) 31. (16 points) T-DNA may be inserted randomly into the plant genome, resulting in mutations in the inserted gene. The process of producing mutants of Arabidopsis induced by this method are as follows: planting wild type Arabidopsis plants, to form buds, on the ground in Agrobacterium (T-DNA with herbicide resistan