能量来源对胎儿宫内发育及胎盘Igf2和H19表达的影响.docVIP

能量来源对胎儿宫内发育及胎盘Igf2和H19表达的影响 生命科学与理学院 生物科学专业 韩兴发 （指导教师：林燕 职称：讲师） 摘要：目的：基于成年疾病的发生与早期胎儿宫内营养密切相关，本试验以目前人类膳食中的主要日粮成分：脂肪、淀粉、纤维作为研究的日粮，探讨不同能量来源对胎盘Igf2、H19基因的表达及胎儿宫内生长的影响。方法：选80只SD母鼠分配到如下四个处理组：高脂肪组、低脂肪组、高脂肪+纤维组、低脂肪+纤维组。在妊娠17.5天屠宰母鼠并称取胎盘和胚胎的重量，采用荧光实时定量PCR技术分析胎盘中印记基因H19和Igf2 mRNA表达水平的差异。结果:低脂+纤维组和高脂+纤维组的Igf2 mRNA 的表达量显著高于高脂和低脂组（P0.05）;高脂+纤维组和低脂组的H19 mRNA表达量显著高于其他两组（P0.05）；高脂肪+纤维组和低脂肪+纤维组的胎儿重量高于高脂肪组和低脂肪组（P0.05）。低脂+纤维组胎盘重量显著高于低脂肪组（P0.05）。结论：在淀粉和脂肪组日粮中添加膳食纤维可调控响胎盘印迹基因Igf2和H19的表达，促进胎盘发育和改善胎盘功能，从而促进胎儿的宫内生长发育。 关键词： H19；Igf2；胎盘；胚胎Effects of dietary energy sources on the fetal growth in uterine and the expression of placenta imprinted gene Igf2 and H19 HAN Xingfa Biological Science ,Grade 2006 Directed by Lin yan (lecturer) Abstract：The incidence of the adult disease is closely associated with the nutritional environment provided for the early embryonic/fetal development, therefore, the objective of the present study is to examine different dietary energy source on the mRNA expression of the imprinted gene Igf2 and H19 and the fetal growth in uterine. 80 SD femal rat were allocated into four dietary treatment, high fat (HF), low fat (LF), high fat plus fiber (HF+f) and low fat plus fiber (LF+f). The rat were slaughtered on day 17.5 of pregnancy, and the weight of placena and foetus were determined. RT-PCR was used to detect the mRNA expression of imprinted gene Igf2 and H19. The results showed that the mRNA expressions of Igf2 in HF+f and LF+f was significantly higher than LF and HF (P0.05). The mRNA expressions of H19 in HF+f and LF was significantly higher than the other two groups (P0.05). The fetal growth in HF+f and LF+f was significantly higher than the other two groups (P0.05), and the placenta weight was higher in LF+f than that in LF (P0.05). In conclusion, supplementation of fiber in starch- or fat- rich diet could promote placenta growth and improve its function so as to progress fetal development in unterine, which could be likely linked with the exp