代沐华李德强韩阳文拉法辛对卒中后抑郁大鼠-中国试验动物学报.doc

研究报告 脑卒中后抑郁大鼠行为学的动态变化及中药补阳还五汤的干预作用 罗 琳1，邓叔华1，易 健1，周赛男1，佘 颜1，刘柏炎1,2 ( 1. 湖南中医药大学，长沙4102082.益阳医学高等专科学校，益阳413000） 【摘要】目的SD大鼠，根据旷场试验基线值, 选择评分相近的60只大鼠随机分为假手术组、卒中组、PSD组、补阳还五汤组、氟西汀组，共5组，每组12只。采用大脑中动脉线栓法建立永久性局灶性脑缺血模型结合CUMS及孤养的方法复制PSD模型。通过体重测量、神经功能评分、糖水消耗实验、旷场实验等指标观察各组大鼠行为学变化。结果 与假手术组、卒中组比较，PSD组大鼠体重和糖水消耗量降低、旷场实验自发活动下降、神经功能缺损加重（P0.05或P0.01）；与PSD组比较，补阳还五汤组和氟西汀组均能增加大鼠体重、糖水消耗量及自发行为，改善其神经功能缺损（P0.05或P0.01）。结论PSD大鼠出现行为学异常，补阳还五汤可以明 【关键词】 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2015.004.000 Dynamic changes of ethology and interventional effect ofa traditional Chinesemedicine,BuyangHuanwu Decoction,inrats with post-stroke depression LUO Lin1, DEN Shu-hua1, YIJian1, ZHOU Sai-nan1,SHE Yan1, LIU Bai-yan1,2 (1.Hunan University of Chinese Medicine, Changsha 410208, China; 2.Yiyang Medical College, Yiyang 413000) 【Abstract】ObjectiveTo observe the effect of a traditional Chinese medicine, BuyangHuanwu Decoction, on the ethology in rat models of post-stroke depression ( PSD).Methods Sixtymale SD rats, after open-field ethology scoring, were randomly divided into five groups ( 12 rats in each group): sham operation group, cerebral ischemic stroke group, post-stroke depression (PSD) group, BuyangHuanwu Decoction group, and fluoxetine group. The ratPSD models were established with separately breeding and chronic unpredictable moderate stress ( CUMS) after middle cerebral artery occlusion. Behavior changes of the rats were detected bybody weight measurement, neurological scoring, sugar consumption test and open-field test. Results Compared with the sham operation group and cerebral ischemic stroke group, the body weight,sugar consumption and open-field spontaneous activity in the PSD model group were decreased, and theneurological function deficitsbecame more severe (P0.01 or P0.05). Compared with the PSD group, Both BuyangHuanwu Decoction group and fluoxet