穿刺液（Puncture solution）.docVIP

穿刺液（Puncture solution） Sample collection and preservation Joint aspiration should be performed by experienced clinicians under strict aseptic procedures. Even if the joint, such as the knee joint, normally contains only 0.1-2ml synovial fluid, it is common to do so unless there is an obvious effusion. Joint cavity puncture and indications are: (1) unknown joint effusion; suspected to be infectious arthritis; searching for pathogenic bacteria; pumping effusion or injecting drugs into the joint cavity, so as to achieve the purpose of treatment. After specimen collection were packed into 3 semi sterile tubes, the first tube made of microbiological examination and the general characters of examination; second pipe heparin per milliliter of synovial 25U heparin sodium for cytological and chemical examination; third tube without anticoagulant to observe whether coagulation. Record extraction quantity at the same time, should not use oxalate and EDTA powder was used as the anticoagulant, because the crystallization of these substances affect the examination of synovial fluid, if the syringe extract only a few little drops, were taken to socialize after submit timely inspection notice immediately. If the relief solution must be preserved, must be centrifuged to remove the cell, because intracellular enzyme release will change the component of synovial fluid. 4 degrees Celsius can be stored for 10 days, when necessary, set negative 20 degrees Celsius under freezing, if not timely testing, complement or enzyme, etc., should be preserved in the negative 70 degrees below the photographer. Two. General character examination 0.1-2ml synovial fluid is difficult to extract in 1. of the normal joint cavities. It does not solidify when placed. Mild inflammation of the yellow color, due to the leakage of red blood cells, hemoglobin free. The bleeding of the puncture injury can be confirmed by the heterogeneity of the liquid in the syringe. The supernatant after the centrifugation is d