细胞染色（Cell staining）.docVIP

细胞染色（Cell staining） 1. peroxidase staining (POX) [normal] cells with blue granules were positive, while large and dense ones were strongly positive. The thin and small ones were weakly negative; no blue particles were negative in the cells. Peroxidase mainly exists in the granulocyte cell. Except for the early granulocyte, all the other stages are positive. The more mature the cell is, the stronger the reaction is. Primary monocytes were negative, immature monocytes, and mature monocytes were weakly reactive. The lymphocyte, megakaryocyte and red cell lines were negative. [] the clinical significance of staining method can be used for the identification of acute leukemia, acute myelogenous leukemia, cells showed positive reaction; multi granular promyelocytic leukemia, showed a strong positive reaction; acute monocytic leukemia, showed weak positive reaction; acute lymphoblastic leukemia. The negative reaction; if positive reaction, the positive rate of 3% ofblast; megakaryocytic leukemia, negative. 2. neutrophil alkaline phosphatase staining (NAP) [normal] the positive rate of healthy people is generally 40%, and the scores are below 70, and the weak ones are the main ones. [clinical significance] (1): in the enhancement of pyogenic infection (Staphylococcus aureus infection is higher than Bacillus), pregnancy, aplastic anemia, acute lymphoblastic leukemia, malignant lymphoma, myelofibrosis, leukemia, malignant tumor, polycythemia vera, estrogen etc.. (2) decrease or no obvious change: acute or chronic myelogenous leukemia, viral or parasitic infection, other types of anemia. 3. specific esterase staining (SE) [result] the ruby granules were positive in cytoplasm. The positive reaction was mainly found in the granulocytes, and the granulocytes were weakly positive, and the promyelocytic cells showed strong positive reaction without increasing with the maturation of granulocytes. Monocytes are generally negative or weakly reactive. Other cells were negative. [clinic